Central mechanism of curcumin on the paraventricular hypothalamic nucleus of high salt induced hypertension rats
10.12092/j.issn.1009-2501.2023.12.005
- Author:
Tingting MENG
1
;
Yifan GAO
1
;
Qinglan ZHANG
1
;
Tingting MENG
2
;
Hongli GAO
2
;
Xiaojing YU
2
Author Information
1. Xi'an Peihua College
2. Xi'an Jiaotong University
- Publication Type:Journal Article
- Keywords:
curcumin;
high salt intake;
hypertension;
hypothalamic paraventricular nucleus
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(12):1357-1364
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To assess the effect of curcumin in hypothalamic paraventricular nucleus (PVN) and mean arterial pressure so as to explore the central mechanism of hypertension. METHODS: Sixty Sprague-Dawley rats which body weights between 170 and 190 grams fed with a normal salt (0.3% NaCl) or a high salt (8% NaCl) diet for 6 weeks. Meanwhile half of each team received curcumin administration or vehicle by intragastric administration. Mean Arterial pressure was measured noninvasively via tail-cuff instrument and their recording system. The PVN tissue samPles were collected and stored at −80 °C for later analyses. We performed the following experimental procedures: Western blot analysis, immunofluorescence, immunofluorescence and statistical analysis. RESULTS:The average arterial blood Pressure of rats in the high-salt diet group was significantly reduced after 6 weeks of curcumin intervention. The levels of NOX2, NOX4, TLR4, MyD88, IL-6, IL-1β, MCP-1 and ROS in the long-term high-salt diet grouP were significantly higher after curcumin intervention. CONCLUSION:Curcumin can improve blood pressure in hypertensive rats induced by long-term high salt, the mechanism may be related to the imProvement of oxidative stress and inflammatory cytokines in the paraventricular nucleus of the hypothalamus.
