Effects of the proliferation, migration and apoptosis of AHVAC - on gastric cancer MKN-28 cells
10.12092/j.issn.1009-2501.2024.03.004
- Author:
Xiaomei HUANG
1
;
Hui ZHI
1
;
Hao CHEN
1
;
Linming LU
1
;
Xiaoqun ZHU
1
;
Lizhen WANG
1
;
Jue ZHOU
1
;
Jinjin PANG
2
;
Jinliang XU
2
Author Information
1. Department of Pathology, Wannan Medical College
2. Clinical Medicine of Wannan Medical College
- Publication Type:Journal Article
- Keywords:
agkis-trodon halys venom antitumor component- (AHVAC-);
cell apoptosis;
cell migration;
cell proliferation;
gastric cancer
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(3):270-276
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of agkis-trodon halys venom anti-tumor component (AHVAC-) on the biological behavior of gastric cancer MKN-28 cells. METHODS: Gastric cancer MKN-28 cells were treated with the experimental concentrations (5, 10, 15 μg/mL) of AHAVC- for 24 h. Cell proliferation and toxicity assay (cell counting kit-8, CCK-8) was used to detect the inhibition rates of the cells in different concentrations of AHVAC-. The migration ability of the cells was evaluated by wound-healing and Transwell assay. The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining, and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence staining. The protein level of Caspease-3 was determined by Western blot. RESULTS: Compared with normal control group, the results of AHVAC- concentration groups showed that with the increase of AHVAC- concentration, the proliferative activity of MN-28 cells decreased gradually (P<0.01), the cell migration ability decreased gradually (P<0.01), and the cell apoptosis rate increased (P<0.05). The expression of apoptosis-related protein Caspease-3 was up-regulated (P<0.01). CONCLUSION: AHVAC- inhibits proliferation and migration of gastric cancer MSN-28 cells and induces apoptosis.
