Effect of quercetin on osteogenic ability of dental pulp mesenchymal cells and repair of calvarial defects in rabbits
10.3969/j.issn.1001-1978.2021.11.011
- Author:
Shao-Guane HU
1
;
Ke-Ke LIU
1
;
Qin-Qin WANG
1
;
Xu HAN
1
;
Yan XU
1
;
Shuang-Ying GUI
2
Author Information
1. School of Stomatology, Anhui Medical University, Affiliated Stomatological Hospital of Anhui Medical University, Laboratory of Anhui Oral Disease Research Center
2. School of Pharmacy, Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
bone graft;
calvarial defect;
mesenchymal stromal cell;
osteogenic differentiation;
quercetin;
tissue engineering
- From:
Chinese Pharmacological Bulletin
2021;37(11):1536-1541
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of quercetin on the osteogenic ability of human dental pulp mesenchymal stromal cells (hDPSCs) in vitro and in vivo. Methods hDPSCs were obtained from the pulp tissues of premolar, and the characteristic surface antigens were identified by flow cytometry. Cell Counting Kit-8 (CCK-8) assay was used to test the cytotoxicity of quercetin. Alkaline phosphatase (A L P) and alizarin red staining were used to detect the osteogenic ability of cells in vitro. The expression of osteogenic genes was detected by qPCR. Four round calvarial bone defects with a diameter of 8 mm were created in 10 male New Zealand rabbits, and they were differentiated and randomized into four groups. Group A, hDPSCs cultured on Bio-Oss
