Effect of Kudinoside D on ox-LDL-induced lipid accumulation and LPS-induced inflammation
10.3969/j.Issn.1001-1978.2021.08.011
- Author:
Chun-Yen BAI
1
;
Da MENG
1
;
Ya-Qiong ZHANG
1
;
Qian ZHOU
1
;
Gao-Xiong RAO
1
;
Yan-Yun CHE
1
;
Wen GU
2
;
Jia-Yu ZHANG
3
Author Information
1. Engineering Laboratory for National Healthcare Theories and Products of Yunnan Province
2. School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine
3. School of Pharmacy, Binzhou Medical University
- Publication Type:Journal Article
- Keywords:
inflammation;
Kudinoside D;
lipid metabolism;
lipopolysaccha- ride;
oxidized low density lipoprotein;
RAW264.7 macrophage cells
- From:
Chinese Pharmacological Bulletin
2021;37(8):1092-1098
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Kudino- side D on lipid accumulation induced by oxidized low density lipoprotein ( ox-LDL) and inflammation induced by lipopolysaccharide ( LPS ) in RAW264.7 cells.Methods Foam cells were established by incubating the RAW264.7 cells with ox-LDL.The concentration of lipid droplets in the cells was observed by oil red staining, and the level of total cholesterol (TC) in cells was measured by enzyme method.The gene and protein expressions of scavenger receptors CD36 and SR-A1, ATP binding cassette transporters A1 and Gl ( ABCA1 and ABCGI) were detected by RT-qPCR and Western blot, respectively.The expressions of inter- leukin-6 (IL-6), interleukin-1 (3 (IL-ip), monocyte chemoattractant protein-1 (MCP-1 ) and tumor necrosis factor-a (TNF-a) were detected by ELISA and RT-qPCR.The protein expressions of mTOR and p-mTOR were detected by Western blot.Results Compared with model group, the high dose of Kudinoside D decreased the content of TC and down-regulated the gene and protein expression of SR-A induced by ox-LDL.Meanwhile Kudinoside D also decreased the levels of IL-ip and MCP-1 and down-regulated the protein expression of p-mTOR induced by LPS.Conclusions Kudinoside D may reduce the intracellular TC content by down-regulating the gene and protein expression of SR-A1.Kudinoside D may play an anti-inflammatory role through mTOR pathway.
