Scutellarin promotes autophagy, inhibits oxidative damage and apoptosis in microvascular endothelial cells during ischemia-reperfusion injury
10.3969/j.issn.1001-1978.2021.07.012
- Author:
Wei-Wei WANG
1
;
Lin SUN
1
;
Tian-Hao BAO
1
;
Hong YE
2
;
Tian-Hao BAO
3
Author Information
1. Cardiology Department, The Second Affiliated Hospital of Kunming Medical University
2. Pathology Department, Kunming Medical University
3. Geriatrics Department, Yunan Psychiatric Hospital
- Publication Type:Journal Article
- Keywords:
apoptosis;
autophagy;
endothelial cells;
ischemia-reperfusion injury;
oxidative injury;
Scutellarin
- From:
Chinese Pharmacological Bulletin
2021;37(7):946-951
- CountryChina
- Language:Chinese
-
Abstract:
Aim: To investigate the effects of Scutellarin (Scu) on microvascular endothelial cells (MEC) with ischemia/reperfusion injury (I/R) and its mechanisms involved. Methods Microvascular endothelial cells were used to build model of I/R, and the protective effects of Scu were observed. Cell viability was determined by MTT assay; SOD. MDA and LDH levels were determined by biochemical method; ICAM-1, VCAM-1, caspase-3 and LC3 mRNA expressions were detected by RT-PCR; LC3, CREB and TLR4 expressions were detected by Western blot. Results MTT assay showed that the cell viability was rescued by 50 μmol • L
