miR-199a-5p regulates DDR1 to inhibit proliferation and migration of human glioma cells
10.3969/j.issn.1001-1978.2021.05.017
- Author:
Zhao-Yue YAN
1
;
Yu-Shuai GAO
1
;
Yu-Long JIA
1
;
Xing-Yao BU
1
Author Information
1. Henan Provincial People's Hospital
- Publication Type:Journal Article
- Keywords:
discoid domain receptor 1;
glioma;
migration;
miR-199a-5p;
proliferation;
U251
- From:
Chinese Pharmacological Bulletin
2021;37(5):687-692
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of miR-199a-5p on the proliferation and migration of human glioma cells. Methods U251 cells were selected as experimental subjects to construct a U251 cell line overexpressing miR-199a-5p. The experiment was divided into; control group (U251 cells without transfection, Control), negative control group (transfected with empty vector plasmid U251 cells, NC) and experimental group (transfected with miR-199a-5p mature mimics, mimics). Real-time fluorescent quantitative PCR was used to detect the expression of miR-199a-5p in each group; CCK-8 was used to detect the proliferation of cells transfected with miR-199a-5p; the cell scratch test and Transwell migration test were used to detect the migration of U251 in each group; Western blot was applied to detect DDR1 expression; a U251 cell line overexpressing DDR1 was constructed to detect the effect of overexpression of DDR1 on the proliferation and migration of U251 cells transfected with miR-199a-5p. Results The level of miR-199a-5p in mimics group was significantly higher than that in control group (P < 0.01), the cell viability was reduced (P < 0.01), and the proliferation ability was weakened (P <0. 01). The expression of DDR1 in miR-199a-5p group cells was significantly reduced (P < 0. 01). Compared with mimincs group, the pcDNA3. 1-DDR1 transfected group could up-regulate DDR1 (P < 0. 01), increase cell viability, and promote cell proliferation (P < 0. 05 or P < 0. 01). Conclusions miR-199a-5p can down-regulate the expression of DDR1 and inhibit the proliferation and migration of human glioma cells.
