Chrysin inhibits LPS-induced autophagy of chondrocytes through PI3K/AKT signaling pathway
10.3969/j.issn.1001-1978.2021.05.013
- Author:
Yang YANG
1
;
Wei HU
1
;
Yu HE
2
;
Yu-Chuan SHI
2
;
Jian-Wei SHU
3
;
Le YU
3
Author Information
1. Dept of Clinical Pharmacology, Anhui Medical University
2. Dept of Gynaecology and Obstetrics, The Second Hospital of Anhui Medical University, Anhui Medical University
3. Dept of Key Laboratory of Anesthesiology and Perioperative Medicine of Anhui Higher Education Institutes, Anhui Medical University
- Publication Type:Journal Article
- Keywords:
autophagy;
chondrocytes;
chrysin;
lipopolysaccharide;
PBK/AKT;
ROS
- From:
Chinese Pharmacological Bulletin
2021;37(5):662-668
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of chrysin on chondrocyte autophagy in rat chondrocyte osteoarthritis model induced by lipopolysaccharide and its mechanism. Methods Normal articular cartilage cells of 10 SPF SD rats were isolated and cultured in vitro, and the autophagy of rat chondrocytes was induced by LPS. The experiment was divided into blank control group, LPS group, chrysin (CHR) group and LPS + CHR group, the activity of cells in each group was detected by CCK-8 method, the mitochondrial membrane potential of cells in each group was detected by Rhodaminel23, and the protein expression of PI3K, AKT, p-PI3K, p-AKT, Beclin-1 and LC3 II in cells of each group was detected by reactive oxygen species, Western blot method of DCFH-DA. Results Chrysin could inhibit the autophagy induced by LPS, especially when the concentration of chrysin was 10 mmol · L
