1, 8-CineoIe improves endothelial cell injury induced by high glucose via regulating autophagy

Xiao-mei Song; Hong Yang; Qiu-shuang Long; Guan-qin Fang; Ge Gao; Shi-quan Gan; Yan Chen; Xiang-chun Shen; Xiao-mei Song; Qiu-shuang Long; Guan-qin Fang; Ge Gao; Shi-quan Gan; Yan Chen; Xiang-chun Shen; Hong Yang

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1, 8-CineoIe improves endothelial cell injury induced by high glucose via regulating autophagy

Author: Xiao-Mei SONG ¹ ; Hong YANG ¹ ; Qiu-Shuang LONG ¹ ; Guan-Qin FANG ¹ ; Ge GAO ¹ ; Shi-Quan GAN ¹ ; Yan CHEN ¹ ; Xiang-Chun SHEN ¹ ; Xiao-Mei SONG ² ; Qiu-Shuang LONG ² ; Guan-Qin FANG ² ; Ge GAO ² ; Shi-Quan GAN ² ; Yan CHEN ² ; Xiang-Chun SHEN ² ; Hong YANG ³
Author Information

1. College of Pharmacy, Guizhou Medical University
2. The High Educational Key Lab of Guizhou Province for Natural Medicinal Pharmacology and Druggabilily
3. Dept of Pharmacy, Maternal and Child Health Hospital of Guiyang
Publication Type:Journal Article
Keywords: 1, 8-Cineole; apoptosis; autophagy; endothelial cells; endothelial dysfunction; high glucose
From: Chinese Pharmacological Bulletin 2021;37(4):472-477
CountryChina
Language:Chinese
Abstract: Aim To investigate the protective effect of 1, 8-Cineole on the injury of human aortic endothelial cells (HAECs) induced by high glucose (HG) via regulating autophagy. Methods Cells were incubated with different doses of 1, 8-Cineole followed by exposing to HG for 60 h, and MTT assay was used to analyse cell viability, lactate dehydrogenase (LDH) was used to detect cytotoxicity, and Western blot was used to detect Beclin1, LC3-II/I, p62, caspase-3 and caspase-9 expressions. Autophagy inhibitor (chloroquine, CQ) was treated on HAECs, and the expressions of Beclinl, LC3-II/I, p62, caspase-3 and caspase-9 were measured by Western blot. Results 1, 8-Cineole increased cell viability, reduced the content of LDH, activated autophagy and inhibited apoptosis. Compared with control group, the expression of Beclinl, LC3-II/I, p62, caspase-3 and caspase-9 in CQ group increased. Simultaneously, the expression of above-mentioned between CQ + HG group and CQ + HG + CH group. Conclusions 1, 8-Cineole has protective effect on the injury of HAECs induced by high glucose, and its effect is related to improving autophagy flux.