Effect of Danggui-Chuanxiong herb pair on JAK-STAT signaling pathway in rats with cerebral ischemia/reperfusion injury
10.3969/j.issn.1001-1978.2021.09.020
- Author:
Wen-Juan NI
1
;
Wen-Hui JIA
1
;
Kun LI
1
;
Jin-Fa JANG
1
;
Wei-Xia LI
1
;
Shu-Qi ZHANG
2
;
Xiao-Yan WANG
2
;
Ming-Liang ZHNAG
2
;
Jin-Fa JANG
2
;
Wei-Xia LI
2
;
Xiao-Yan WANG
3
;
Jin-Fa JANG
3
;
Wei-Xia LI
3
Author Information
1. College of Pharmacy, Henan University of Chinese Medicine
2. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, The First Affiliated Hospital of Henan University of Chinese Medicine
3. Provincial and Ministerial Co-Construction Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine, Henan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
action mechanism;
brain protection;
cerebral ischemia/reperfusion;
Danggui-chuanxiong herb pair;
JAK-STAT signaling pathway;
promote blood circulation and remove blood stasis
- From:
Chinese Pharmacological Bulletin
2021;37(9):1305-1311
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the regulatory effect of Danggui-chuanxiong herb pair (GX) on JAK-STAT signaling pathway in rats with cerebral ischemia/reper-fusion injury (I/R). Methods The I/R injury rat model was constructed by modified suture occlusion method. After 24 hours of perfusion, Zea Longa scoring method was used to score the neurological function, TTC staining to detect the cerebral infarct volume of rats, HE staining to observe the pathological changes of brain tissues, the biochemical method to determine the MDA, SOD, GSH-Px expression, ELISA to detect the expression of NF-κB, VEGF, ICAM-1 and PAH in brain tissues, and immunohistochemical method to detect JAK2, p-STAT3, AKT And ERK1/2 expression of the brain tissue ischemic penumbra area. Results Compared with sham group, model rats had severe neurological damage, larger cerebral infarction, necrosis, edema, inflammation, disorder of nerve cell arrangement, abnormal cell enlargement, vacuole-like changes, neuron reduction and other pathologies in brain tissues. The expression JeveJs of MDA, NF-κB, VEGF, PAI-1 and ICAM-1 in brain tissues of model group significantly increased, and the expression levels of GSH-Px and SOD were significantly reduced. Compared with model group, the neurological scores of rats in GX
