Inhibition of YAP-c-Myc signaling pathway involved effect of ORI on anti-glioma
10.3969/j.issn.1001-1978.2021.03.019
- Author:
Chen WANG
1
;
Shuang CHEN
1
;
Xiao-Jiao MA
1
;
Huan XIAO
1
;
Hong-Mei QIU
1
Author Information
1. Chongqing Key Lab of Biochemistry and Molecular Pharmacology, Dept of Pharmacology, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
C-Myc;
Migration;
Oridonin;
Proliferation;
U87 glioma cells;
YAP
- From:
Chinese Pharmacological Bulletin
2021;37(3):403-409
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of oridonin on proliferation, migration and apoptosis of U87 glioma cells and to explore the involvement of the mechanism in the inhibition of Yes-associated protein (YAP)-c - Myc signaling pathway. Methods The effect of oridonin on U87 viability was measured by MTT assay; the migration and invasiveness of cells were measured by transwell assays; the apoptotic rates of cells were assessed by flow cytometry; the caspase-3, B c l - 2, Bax, YAP, c - MycmRNA expression in U87 glioma cells was detected by real-time quantitative P C R; the caspase-3, Bcl-2, Bax, YAP, p-YAP (Seri 27), c-Myc protein expressions were detected by Western blot. Results The proliferation of U87 cells was significantly inhibited by oridonin in a dose-dependent manner (P < 0. 05), and the ability of cell migration and invasion was weakened (P <0. 01), cell apoptosis rate in flow cytometry analysis increased significantly (P <0. 01), the protein and mRNA expression of caspase-3 increased (P < 0. 05), the mRNA and protein expression of Bcl-2/Bax decreased (P < 0. 05), the mRNA and protein expression of Y A P and c-Myc decreased (P < 0. 05), and the protein expression of p - Y A P increased (P < 0. 05). Conclusions Oridonin can significantly inhibit the proliferation and migration of U87 glioma cells and promote the transformation apoptosis of glioma cells; the mechanism may be related to the inhibition of YAP-c-Myc signaling pathway.
