Homoharringtonine activates ATM/p53 pathway to inhibit proliferation of human liver cancer cell PLCS

Author: Yu-Mei DU ¹ ; Jia-Feng TANG ² ; Shi-Ying HUANG ² ; Tao ZHANG ² ; Rui-Jing LU ² ; Shuang HE ² ; Hang XU ² ; Jing LI ² ; Di-Long CHEN ² ; Jia-Feng TANG ³ ; Tao ZHANG ³ ; Di-Long CHEN ³ ; Tao ZHANG ⁴ ; Hang XU ⁴ ; Jian-Hua RAN ⁴
Author Information

1. College of Public Health and Management, College of Basic Medicine, Chongqing Medieal University
2. Lab of Stem Cells and Tissue Engineering, College of Basic Medicine, Chongqing Medieal University
3. Chongqing Three Gorges Medical College
4. Center of Neuroscience Research, College of Basic Medicine, Chongqing Medical University
Publication Type:Journal Article
Keywords: CDK2; DNA damage; Hepatocellular carcinoma; Homoharringtonine; P53; PLC5
From: Chinese Pharmacological Bulletin 2021;37(3):380-385
CountryChina
Language:Chinese
Abstract: Aim To explore the effect of homoharringtonine (HHT) on the prohferation of liver cancer cell PLCS and its possible mechanism. Methods CCK-8 and EdU were used to detect the effect of HHT on the proliferation of PLCS cells; flow cytometry was employed to assess the effect of HHT on cell cycle of PLCS; Western blot was applied to measure the expression levels of cycle-related proteins cyclinA, CDK 2, p 2 1, p53 and A T M. Results Treated with HHT (0, 5, 10, 20, 40, 80 • L