Mechanism of Fluspirilene suppressing HCC through decreasing Akt expression
10.3969/j.issn.1001-1978.2021.03.011
- Author:
Xi-Nan SHI
1
;
Zhuo-Ran MENG
1
;
Shan ZHANG
1
;
Xi-Nan SHI
2
;
Wei CHEN
2
;
Rong-Ping ZHANG
3
;
Yu-Huan XIE
4
;
Yu-Huan XIE
5
;
Shi-Guo WU
6
;
Rong CHEN
7
;
Wei-Ping WAN
8
;
Jian WANG
9
Author Information
1. School of Basic Medical Sciences, Yunnan University of Chinese Medicine, Dept of Pathology,
2. Southwest Guizhou Vocational and Technical College for Nationalities, Dept of Medicine,
3. Institute of Southern Chinese Medicine, School of Chinese Medicine, Yunnan University of Chinese Medicine
4. Yunnan Provincial University Key Laboratory of Aromatic Chinese Herb Research, Yunnan University of Chinese Medicine
5. Yunnan Innovation Team of Application Research on TCM Theory of Prevention Disease
6. Depl of Teaching and Research of Formulas of Chinese Medicine
7. Dept of Physiology .School of Basic Medical Sciences, Yunnan University of Chinese Medicine
8. Kunming Municipal Hospital of Traditional Chinese Medicine, Dept of Anorectal,
9. The First Affiliated Hospital of Yunnan University of Chinese Medicine, Dept of Neurology,
- Publication Type:Journal Article
- Keywords:
Akt;
CDK2;
cell cycle;
fluspirilene;
hepatic cell carcinoma;
mRNA array
- From:
Chinese Pharmacological Bulletin
2021;37(3):356-361
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanism of Fluspirilene inhibiting HCC through decreasing the expression of Akt.Methods The difference of mRNA was verified by the test of protein expression between Fluspirilenc treatment group and control group by HCC experiment in vivo and vitro, including Western blot, IHC after mRNA array.Results Akt expression was lower in Fluspirilene treatment group than that in control group by mRNA array.Protein expression of Akt, phosphorylate-CDK2 and phos- phorylate-Rb decreased massively in Fluspirilene treatment group in a concentration-dependent manner in HepG2 and Huh7 cells by Western blotting compared with those in control group.Declined expression of phosphorylate-Akt was proved in a concen- tration-dependent manner in xenograft tumor tissues in Fluspirilene treatment group compared with that in control group in IHC test.Conclusions Fluspirilene inhibits HCC by decreasing significantly the protein expression of Akt, phosphorylat-Akt, phos- phorylate-CDK2 and phosphorylate-Rb.
