Correlation between gut microbiota and FGF21 in mice with insulin resistance
10.3969/j.issn.1001-1978.2021.03.008
- Author:
Ye WANG
1
;
Xue HAN
1
;
Xiao-Ting SHA
1
;
Chan-Yue WANG
1
;
Lin-Lin LI
1
;
Pei-Pei ZHANG
2
;
Lin-Lin LI
3
Author Information
1. School of Pharmacy, Xinjiang Medical University
2. School of Pharmacy, Heze University
3. Xinjiang Medical University Jointly Established State Key Luib of High Incidence Causes Arul Prevention in Centred Asia
- Collective Name:School of Pharmacy, Xinjiang Medical University
- Publication Type:Journal Article
- Keywords:
3- Klotho;
FGF21;
FMT;
gut microbiota;
insulin resistance;
RT-qPCR
- From:
Chinese Pharmacological Bulletin
2021;37(3):337-342
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of normal glucose tolerance fecal microbiota transplantation ( FMT) on the gut microbiota in mice with insulin resistance(IR) and its correlation with FGF21 , and to explore the possible mechanisms of gut microbiota affecting IR.Methods After the establishment of IR model with high-fat diet feeding, 30 successful IR model mice were randomly divided into three groups; insulin resistance ( IR ) group, IR + metformin( Met) group, and IR + glucose tolerance normal fecal microbiota transplantation (FMT)group, and blank control(Control) group, with 10 mice in each group.After eight weeks of administration, the body mass and fasting blood glucose of mice at 8th week were recorded, then the number of target bacteria in fecal samples and the mRNA expression levels of FGF21 and its receptors in liver, colon and ileum tissues were detected by Real-time quantitative PCR( RT-qPCR).Results ® Compared with control group, the body mass and fasting blood glucose increased in IR group mice, while the mRNA expression levels of FGF21/p-Klolho/FGFRl/FGFR4 in liver, colon and ileum tissues were down-regulated.The levels of Bacteroules and R.sarlorii were reduced in fecal samples, and the levels of P.distasonis, M.schaedleri and R.gnavus increased.These indices were reverted by Met and FMT treatment.(2) The expression of FGF21 was negatively correlated with FBG, P.distasonis , M.schaedleri and R.gnavus, and positively correlated with Bacteroides and B.sartorii.Conclusions FMT can increase the expression level of FGF21 and regulate gut microbiota, and the two are closely related , which may be one of the important mechanisms of FMT in improving insulin resistance.
