Study on ulcerative colitis activity and its chemical constituents in effective part of Gardeniae Fructus

Chao Yang; Jing Liu; Rui Zhong; Zhi-gui WU; Jian-guo Pei; Yao Chen; Xiao Huang; Sha Gao; Rui Kan; Xiao-mei FU; Zhi-gui WU; Xiao-mei FU

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Study on ulcerative colitis activity and its chemical constituents in effective part of Gardeniae Fructus

Author: Chao YANG ¹ ; Jing LIU ¹ ; Rui ZHONG ¹ ; Zhi-Gui WU ¹ ; Jian-Guo PEI ¹ ; Yao CHEN ¹ ; Xiao HUANG ¹ ; Sha GAO ¹ ; Rui KAN ¹ ; Xiao-Mei FU ¹ ; Zhi-Gui WU ² ; Xiao-Mei FU ²
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1. School of Pharmacy of Jiangxi University of Traditional Chinese Medicine
2. Fan Cuisheng Studio of National Famous Traditional Chinese Medicine,
Publication Type:Journal Article
Keywords: extract; Gardeniae Fructus; MPO; SOD; ulcerative colitis; UPLC-MS/MS
From: Chinese Pharmacological Bulletin 2021;37(2):263-270
CountryChina
Language:Chinese
Abstract: Aim To study the therapeutic effect of 35% and 70% ethanol elution sites of Gardeniae Fructus extract on 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol induced ulcerative colitis (UC) in rats, and to identify the chemical composition of the active elution site using mass spectrometry. Methods The UC model induced by TNBS was used in rats, and the different eluted parts were administered by gavage at a dose of 2. lg/kg for 7 days. Body weight measurement , disease activity index (DAI) score, and pathological score of colon tissues were compared. Myeloperoxidase (MPO) , superoxide dismutase (SOD), malondialdehyde ( MDA ) , nitric oxide ( NO ) , tumor necrosis factor-a (TNF-a) , interleukin in mouse colon tissue -6 (IL-6), interleukin-1 (3 (IL-ip) levels were compared among groups. Liquid-mass spectrometry was used to identify the chemical components of the parts with better efficacy. Results Compared with model group, the weight loss in 35% elution site group was significantly improved, the DAI and histopathology scores were markedly reduced, and the contents of MPO, NO, MDA, TNF-a, IL-6 and IL-1(3 in tissues were apparently reduced. SOD content increased significantly (P <0. 01). A total of 19 chemical components were identified by LC-MS, 11 of which were iri- doids. Conclusions The 35% elution site of Gardeni- a has obvious therapeutic effect on UC rats, and the iridoid component may be the material basis for its function.