HBEGF up-regulate expression level of PD-L1 in glioblastoma cells partly through LIF
10.3969/j.issn.1001-1978.2021.11.019
- Author:
Bo LIANG
1
;
Xin-Jun WANG
1
;
Jian-Ye WANG
1
;
Shao-Long ZHOU
1
;
Zhuo YANG
1
;
Yang ZHAO
2
Author Information
1. Dept of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University
2. Dept of Pharmaceutics, The Fifth Affiliated Hospital of Zhengzhou University
- Publication Type:Journal Article
- Keywords:
glioma cells;
heparin-binding epidermal growth factor;
knockout;
leukemia inhibitory factor;
mechanism;
programmed death ligand 1
- From:
Chinese Pharmacological Bulletin
2021;37(11):1585-1592
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanism of heparin-binding epidermal growth factor (HBEGF) in upregulating the expression of programmed death ligand 1 (PD-L1) in glioma cells by regulating leukemia inhibitory factor (LIF). Methods Pearson analysis was used to analyze the correlation between HBEGF, LIF and PD-L1 mRNA in primary gliomas, U251 and U87 cells were treated with HBEGF neutralizing antibody and HBEGF was added after HBEGF knockout, the mRNA and protein levels of LIF and PD-L1 were detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot, HBEGF knockout cells were added with LIF neutralizing antibody on the basis of adding HBEGF, and the levels of PD-L1 mRNA and protein were detected. Results Pearson analysis showed that HBEGF, LIF, and PD-L1 mRNA were positively correlated (P < 0.05). After adding HBEGF neutralizing antibody to U251 and U87 cells, the mRNA and protein levels of LIF and PD-L1 gradually decreased with cell passage (P < 0.05). After HBEGF knockout, the mRNA and protein levels of LIF and PD-L1 decreased (P < 0.05), and the mRNA and protein levels of LIF and PD-L1 increased after HBEGF was added (P < 0.05). In HBEGF knockout cell lines, the levels of PD-L1 mRNA and protein were up-regulated after HBEGF was added (P<0.05), and the levels of PD-L1 mRNA and protein was down-regulated by LIF neutralizing antibody (P < 0.05). Conclusion The partial induction of LIF by HBEGF in glioblastoma is an intermediate process for HBEGF to maintain PD-L1 expression.
