Propofol protects against alcohol-induced liver injury to up-regulate mitophagy via SIRT2
10.3969/j.issn.1001-1978.2021.11.007
- Author:
Wen-Ting XUAN
1
;
Wen-Ting XUAN
2
;
Xin-Yi LU
2
;
Jun LI
2
Author Information
1. Dept of Anesthesiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
2. School of Pharmacy, Anhui Medical University
- Publication Type:Journal Article
- Keywords:
alcoholic liver disease;
liver protection;
mitophagy;
perioperative liver protection;
propofol;
SIRT2
- From:
Chinese Pharmacological Bulletin
2021;37(11):1512-1518
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanisms that propofol inhibits alcohol-induced liver injury in C57 mice. Methods HE and oil red staining methods were used to measure the injury and lipid accumulation rates in liver. Immunofluorescence (IF) was used to identify the colocalization rate of mitochondria and LC3. ALT and AST tests were used to identify the liver injury level. Molecular docking experiment was used to predict the target protein of propofol. Western blot and immunohistochemistry (IHC) experiments were used to detect SIRT2 protein level. Results Propofol could significantly attenuate alcohol-induced high ALT/AST level, lipid accumulation, and enhance mitophagy level. According to molecular docking and Western blot experiments, propofol had high correlation with SIRT2 protein, and propofol could rescue alcohol-induced low expression level of SIRT2 protein. In addition, sirReal2 (an inhibitor of SIRT2) could significantly inhibit the protective effects of propofol in mouse liver. Con-clusion Propofol could protect against alcohol-induced liver injury through SIRT2 protein to enhance mitophagy level.
