The protective effect of miR-5088-5p on hepatocyte pyroptosis induced by homocysteine

Author: Xiao-Yan DONG ¹ ; Da-Yue LIU ¹ ; Ling-Bo XU ¹ ; Yi-Deng JIANG ¹ ; An-Ning YANG ¹ ; Gui-Zhong LI ¹ ; Xiao-Yan DONG ² ; Da-Yue LIU ² ; Ling-Bo XU ² ; Ling-Yu GU ² ; Yi-Deng JIANG ² ; An-Ning YANG ² ; Yun JIAO ² ; Gui-Zhong LI ² ; Xiao-Yan DONG ³ ; Da-Yue LIU ³ ; Ling-Bo XU ³ ; Ling-Yu GU ³ ; Yi-Deng JIANG ³ ; An-Ning YANG ³ ; Yun JIAO ³ ; Gui-Zhong LI ³ ; Ling-Yu GU ⁴ ; Yun JIAO ⁵
Author Information

1. School of Basic Medical Sciences, General Hospital, Ningxia Medical University
2. NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, General Hospital, Ningxia Medical University
3. Ningxia Key Lab of Vascular Injury and Repair Research, General Hospital, Ningxia Medical University
4. Clinical Medical College, General Hospital, Ningxia Medical University
5. Dept of Infectious Diseases, General Hospital, Ningxia Medical University
Publication Type:Journal Article
Keywords: CBS mice; hepatocyte; hyperhomocysteinemia; miRNA; miRNA-5088-5p; pyroptosis
From: Chinese Pharmacological Bulletin 2021;37(11):1565-1570
CountryChina
Language:Chinese
Abstract: Aim To explore the role of miR-5088-5p in hepatocyte pyroptosis induced by homocysteine. Methods Hepatocytes were cultured and divided into control group and Hcy group. After transfected miR-5088-5p NC and miR-5088-5p mimic under Hcy treatment, the expression of NLRP3, Caspase 1 and IL-1β was detected by Western blot. The expression of miR-5088-5p was detected by RT-qPCR. CBS