Salvianolic acid A inhibits platelet and neutrophil activation
- Author:
Wen-Jie YANG
1
;
Lu-Lu MA
1
;
Le-Yu FANG
1
;
Chun-Xiao LI
1
;
Lu-Sha ZHANG
1
;
Li-Yuan ZHANG
1
;
Qian-Yi WANG
1
;
Wei SUN
1
;
Yu-Ze LENG
1
;
Yue-Jin XUE
1
;
Meng-Yao LI
1
;
Hong WANG
1
;
Hong-Ying WANG
2
;
Lu CHEN
3
Author Information
- Publication Type:Journal Article
- Keywords: complement C3aR; myocardial infarc¬tion; neutrophils; platelet activa-tion; platelet recruitment; Salvianolic acid A
- From: Chinese Pharmacological Bulletin 2022;38(5):740-748
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effeets of Salvianolie aeirl A (SAA) on platelet recruitment, activation and neutrophils in heart of myocardial infarction ( Ml ) mice.Methods C57BL/6 mice were randomly divid¬ed into: Sham operation group.Ml model group, SAA (5, 10 mg • kg 1 ) group, tirofiban (Tirofiban, 0.87 mg • kg ' ) group, using tail vein injection for 3 d.Echocardiography and HE staining were used to detect mouse heart function and infarct area; 1HC, FCS, ELISA, Western blot and other methods were used to explore the inhibitory effect of SAA on platelet and neutrophil activation.Results Compared with Ml group, SAA could improve the cardiac function and cardiac physiology changes of Ml mice, reduce the ex¬pression of CD42c in myocardial tissue and CD62p in peripheral blood without affecting tail bleeding time, reduce ADP-induced platelet activation and increase p- VASP/VASP ratio, reduce the ratio of p-PI3K/PI3K and p-AKT/AKT, reduce the expression of CD45, Ly6G, CXCL1 and CXCL2 in myocardial tissue, re¬duce the expression of complement component C3aR in myocardial tissue, and reduce C3a-induced NE and MPO, MMP9, LF level.Conclusions SAA has an anti-platelet activation effect by inhibiting the PI3K/ AKT and VASP pathways and an anti-neutrophil acti¬vation effect by inhibiting the expression of C3aR and C3a.
