Role of calcium-independent phospholipase A2 in contraction of intrarenal artery smooth muscle

Xiao-yue Qin; Sui-min LI; Shu-zhen Chen; Yi-rong Wang; Chun-yu Deng; Xiao-yue Qin; Sui-min LI; Peng Zeng; Shu-zhen Chen; Yi-rong Wang; Su-juan Kuang; Hui Yang; Fang Rao; Chun-yu Deng; Peng Zeng; Chun-yu Deng; Su-juan Kuang; Hui Yang; Fang Rao; Chun-yu Deng

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Role of calcium-independent phospholipase A2 in contraction of intrarenal artery smooth muscle

Author: Xiao-Yue QIN ¹ ; Sui-Min LI ¹ ; Shu-Zhen CHEN ¹ ; Yi-Rong WANG ¹ ; Chun-Yu DENG ¹ ; Xiao-Yue QIN ² ; Sui-Min LI ² ; Peng ZENG ² ; Shu-Zhen CHEN ² ; Yi-Rong WANG ² ; Su-Juan KUANG ² ; Hui YANG ² ; Fang RAO ² ; Chun-Yu DENG ² ; Peng ZENG ³ ; Chun-Yu DENG ³ ; Su-Juan KUANG ⁴ ; Hui YANG ⁴ ; Fang RAO ⁴ ; Chun-Yu DENG ⁴
Author Information

1. School of Biological Science and Engineering, South China University of Technology
2. Medical Research Center of Guangdong General Hospital, Guangdong Provincial Key Laboratory of Clinical Pharmacology, Guangdong Academy of Medical Sciences ,
3. School of Medicine, South China University of Technology
4. Cardiovascular Internal Medicine of Guangdong Cardiovascular Institute
Publication Type:Journal Article
Keywords: arachidonate-regulated calcium channel; bromoenol lactone; calcium-independent phospholipase A2; intrarenal artery; store-operated calcium channel; vascular tone
From: Chinese Pharmacological Bulletin 2022;38(5):733-740
CountryChina
Language:Chinese
Abstract: Aim To investigate the role of calcium-independent phospholipase A2(iPLA2)in calcium regu-lation of intrarenal artery smooth muscle contraction.Methods The method of measuring the tension of isolated arterioles was used to explore the effect of bromoenol lactone(BEL), a specific inhibitor of iPLA2, on the tension of the intrarenal arteries in mice induced by different calcium channels, and the laser confocal calcium measurement technology was used to investigate the effect of BEL on the intracellular calcium influx mediated by arachidonic acid-mediated calcium channels.Results The intrarenal artery concentration dependent contractile response induced by the vasoconstrictors phenylephrine and 5-hydroxy tryptamine was inhibited by BEL(P<0.01).The contraction curve induced by CaCl2 was also inhibited by BEL(P<0.05).In the calcium-free K-H solution incubated with nifedipine, the intrarenal artery vasoconstriction caused by the release of sarcoplasmic reticulum calcium and the calcium influx of the SOC channel induced by CaCl2 was inhibited by BEL(P<0.05).BEL significantly inhibited the external calcium influx mediated by the ARC channel of human aortic smooth muscle cell lines incubated with nifedipine(P<0.01).Conclusions iPLA2 mediates the contractile response of intrarenal arteries by regulating the functions of L-type calcium channels, sarcoplasmic reticulum calcium release, SOC channels and ARC channels.