Mechanism of cornus officinalis polysaccharide on proliferation and apoptosis of gastric cancer cells
10.3969/j.issn.1001-1978.2022.02.013
- Author:
Zhi-Yong NIE
1
;
Shou-Miao LI
1
;
Wei-Jie WANG
1
;
Huan-Huan LI
1
;
Dong-Rlong YUAN
1
Author Information
1. Anyang Cancer Hospital, Dept of Oncology
- Publication Type:Journal Article
- Keywords:
autophagosome;
autophagy;
Beclin1;
LC3B-Ⅱ;
liver cancer;
nifedipine
- From:
Chinese Pharmacological Bulletin
2022;38(2):222-227
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of nifedipine on the formation of autophagosomes in hepatoma cell line Huh-7 and its mechanism.Methods Different concentrations of nifedipine were used to interfere with the proliferation of Huh-7 cells in vitro.The effect of nifedipine on the proliferation of Huh-7 cells was detected by cell proliferation experiment and colony formation experiment.The expressions of Beclin1 and LC3B-Ⅱ were detected by Western blot.The effect of nifedipine on the formation of autophagosomes in Huh-7 cells was observed by laser scanning confocal microscopy.Results Nifedipine significantly inhibited the proliferation of Huh-7 cells in a time-and concentration-dependent manner.The IC50 of nifedipine on day 2 was 22.7 mg·L-1.Nifedipine at the concentration of 25 mg·L-1 significantly reduced the colony formation rate of Huh-7 cells compared with the control group, and the inhibition rate of colony formation was(95.46±0.45)%.Western blot analysis showed that nifedipine significantly up-regulated the protein expression levels of Beclin1 and LC3B-Ⅱ.The amount of autophagosomes in nifedipine group cells were more than that of control group, which was observed by laser scanning confocal microscopy.Conclusions Nifedipine significantly inhibits the proliferation of Huh-7 cells and promotes the formation of autophagosomes, which may be related to the up-regulation of Beclin1 protein expression by nifedipine.
