Paeonol regulates phenotypic conversion of macrophages via estrogen receptor-a
10.3969/j.issn.1001-1978.2022.01.008
- Author:
An Na-Qi LI
1
;
Zi-Han ZHU
1
;
Fan-Zhang YIN
1
;
Wen-Wen LI
1
;
Zhe-Yu ZHOU
1
;
Zhong-Yang WANG
1
;
Yu LI
1
;
Xiang WU
2
;
Hui-Min BIAN
2
Author Information
1. School of Medicine and Holistic Integrative Medicine
2. School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
estrogen receptor;
lipopolysac- charide;
macrophage;
paeonol;
phenotypic transformation
- From:
Chinese Pharmacological Bulletin
2022;38(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of paeonol on macrophage phenotvpe conversion based on estrogen receptora (ERa).Methods The macrophage Ml polarization model was established by 100 jjig • L"' LPS and 20 pug • L_1 I FN-7.ELISA was used to examine the effects of paeonol on tumor necrosis factor-a ( TNF-cx ) , interleukin-1 £ ( 1L-1 £ ) , interleukin-10 (IL-10), superoxide dismutase (SOD) , and malondi- aldehyde ( MDA).Western blot was used to detect the expression of M1 phenotvpe markers iNOS, CD86 and M2 phenotvpe markers Arg-1 and CD 163 in macrophages.Further, the methods of blockers and shRNA interference were used to verify whether the effect of paeonol was mediated by ERa.Results ELISA results shower] that paeonol reduced the content of TNF-a, IL- lp and MDA, and increased the content of IL-10 and SOD.Western blot results showed that paeonol reduced the expression of iNOS and CD86 proteins in model group, and increased the expression of Arg-1 and CD163 proteins.Both ERa selective blocker MPP and ERa shRNA reduced the efficacy of paeonol, while ERp selective blocker PHTPP had no significant effect on paeonol.Conclusion Paeonol can induce the transformation of macrophages into M2 type by ERa and alleviate the progression of atherosclerosis.
