Mechanism of Huoxue Rongluo recipe in promoting angiogenesis after ischemic stroke based on correlation between miR-370-3p and JAK2/STAT3 pathway
10.3969/j.issn.1001-1978.2022.02.024
- Author:
Cui-Lan GONG
1
;
Ren-Yi YANG
1
;
Xing-Ying FU
1
;
Jun-Xi LI
1
;
Cui-Lan GONG
2
;
De-Sheng ZHOU
3
;
Jia LING
4
Author Information
1. Hunan University of Traditional Chinese Medicine
2. Changde Hospital Affiliated to Hunan University of Traditional Chinese Medicine
3. The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine
4. Cultiv. Base of State Key Lab. Jointly Built by Hunan Prov. and Min. of Traditional Chinese Med. Powder and Innov. Med.
- Publication Type:Journal Article
- Keywords:
angiogenesis;
butylphthalide;
Huoxue Rongluo recipe;
ischemic stroke;
JAK2/STAT3 pathway;
miR-370-3p;
Stattic
- From:
Chinese Pharmacological Bulletin
2022;38(2):297-304
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanism of Huoxue Rongluo reeipe in promoting angiogenesis after ischemic stroke based on the correlation between mir-370-3p and JAK2/STAT3 pathway.Methods Hats were randomly divided into six groups.MCAO/R method was used to establish the model.After seven days of intragastrie administration,the expressions of CD31 ,vWF and vascular endothelial growth factor ( VEGF) in brain tissue were observed by immunofluorescence stai- ning; the expression of JAK2 ,p-jak2,STAT3 and p- STAT3 in brain tissue was detected by Western blot; J the expressions of JAK2,STAT3 mRNA and mir-370- 3p in brain tissue were detected by real-time PCR f RT-PCR) ; the correlation between mir-370-3p and JAK2/STAT3 pathway was analyzed by Pearson correlation ; the expressions of lncma-hl9 and mir-370-3p were detected by real-time quantitative PCR ( RT qPCR) ; the targeting relationship between lncrna-hl9 and mir-370-3p was detected by luciferase reporter assay.Results Huoxue Rongluo decoction could increase the microvessel density and average fluorescence intensity of VEGF,up-regulate JAK2 and STAT3 mR- NA,down-regulate the expression of mir-37()-3p,and promote the expressions of JAK2,p-jak2 ,STAT3 and p- STAT3.Mir-370-3p was highly negatively correlated with JAK2 and STAT3 mRNA respectively,which could be reversed by stattic,an inhibitor of STAT3 SH2 domain.Conclusions Huoxue Rongluo recipe may stimulate angiogenesis after ischemic stroke by down- regulating the expression of mir-370-3p,activating JAK2 / STAT3 pathway and promoting the expression of downstream VEGF,so as to improve the symptoms of neurolo.
