The effects of the recommended prescription for COVID-2019-lung-spleen qi deficiency on in vitro SARS-CoV-2 replication and pro-inflammatory factors expression
- Author:
Pei-Fang XIE
1
;
Hong-Mei LI
1
;
Yu-Lanl SHAO
1
;
Yue FANG
1
;
Zhi-Li SHEN
1
;
Shu-Wei DONG
1
;
Xue-Shan XIA
1
;
Hui LI
2
;
Rong-Tao LI
2
;
Hong-Jun YANG
2
;
Qin-Hai MA
3
;
Fang LI
3
;
Zi-Feng YANG
3
;
Jin-Cun ZHAO
3
Author Information
- Publication Type:Journal Article
- Keywords: anti-inflammation; anti-virus; common coronaviruses; SARS-CoV-2; The recommended prescription of COVID-2019 lung-spleen qi deficiency 4-1
- From: Chinese Pharmacological Bulletin 2022;38(3):460-469
- CountryChina
- Language:Chinese
- Abstract: Aim To determine the antiviral and anti-inflammatory effects of the recommended prescription for COVID-2019-lung-spleen qi deficiency(4-1)against in vitro infection of SARS-CoV-2 and common coronaviruses.Methods The main chemical substances of 4-1 were analyzed by LC-MS.The toxicity and antiviral effects of of 4-1 were detected by MTT and by CPE assay, respectively.The viral loads in cell supernatant and the expression of inflammatory factors induced by viral infection were determined by qRT-PCR.Results The recommended prescription 4-1 contained 94 chemical compounds, including flavonoids, steroids, sesquiterpenoids, and so on.The range of selection indexes for SARS-CoV-2 and common coronaviruses was 8.44±0.4952.26±2.3.This prescription could inhibit the proliferation of SARS-CoV-2, the expression of ACE2 and S mRNA, and down regulate IL-1α and CCL-5/RANTES at 10, 5, and 2.5 g•L-1 doses.Further, at doses of 20, 10 and 5 g•L-1, it could inhibit the proliferation of three common coronaviruses and suppress the overexpression of IL-6, CXCL-8/IL-8, CXCL-10/IP-10, TNF-α, IFN-α, CCL-2/MCP-1, MIG and CCL-5/RANTES induced by OC43/229E infection.The inhibitory effects were dose-dependent.Conclusions The prescription 4-1 has antiviral and anti-inflammation effects against multiple coronaviruses.This study provides the research basis for the treatment of common respiratory viral infections and emerging infectious diseases such as COVID-19 by using traditional Chinese medicine.
