Piezol involved in electrical remodeling in atrial myocytes induced by high hydrostatic pressure through Src kinase
- Author:
Yuan FANG
1
;
Qian LI
1
;
Guan-Hao LUO
1
;
De-Wei PENG
1
;
Lu-An LI
1
;
Qiao-Qiao LI
1
;
Xue-Shan LUO
1
;
Chun-Yu DENG
1
;
Yu-Mei XUE
1
;
Fang RAO
1
;
Shu-Lin WU
1
Author Information
- Publication Type:Journal Article
- Keywords: atrial fibrillation; electrical remodeling; high hydrostatic pressure; L-type calcium current; Piezol protein; Src kinase
- From: Chinese Pharmacological Bulletin 2022;38(3):422-428
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the role of mechano- sensitive ion channel Piezol in regulating electrical re-modeling of atrial myocytes induced by hypertension and to further explore the potential mechanisms.Methods Spontaneously hypertensive rats ( SHR ) aged 30 - 32 weeks treated with or without valsartan (30 mg • kg 1 • d 1 ) were used.Wistar rats were used as control.Western blot was used to detect the protein expression of Piezol , Src and Cavl.2 in atrial appendages of rats and in atrial myocytes ( HL-1 cells) exposed to different levels of high hydrostatic pressure (20 and 40 mmHg) , Piezol inhibitor (GsmTx4) and agonist ( Yodal ) in vitro.Whole-cell patch clamp technique was employed to detect L-tvpe calcium current (ICa, ) and action potential duration ( APD) of atrial myocytes.Results Compared with Wistar rats in control group, the protein expressions of Piezol and Src significantly increased and the expression of Cavl.2 decreased in SHR group (P < 0.05 ), while the a- bove changes could he reversed in SHR treated with valsartan( P < 0.05 ) .Meanwhile, higher hydrostatic pressure (40 mniHg) could increase the expressions of Piezol and Src in HL-1 cells( P <0.05) and decrease the protein expression of Cavl.2 (P <0.05 ) , accompanied by a shortened APD and a decreased ICa,.GsmTx4 could significantly reverse the above changes.In addition, Piezol agonist Yodal could simulate electrical remodeling and related signal molecule changes in atrial myocytes induced by the high hydrostatic pressure.Conclusions Mechanosensitive ion channel Piezol participates in electrical remodeling induced by hypertension via activating Src kinase signaling pathway and then leading to the decrease of ICa ,.
