Honokiol inhibits IL-1 induced angiogenesis in human lung cancer cell H460 through NF-kB signaling pathway
- Author:
Xu-Dong CHENG
1
;
Ting CHEN
1
;
Meng UN
1
;
Ting CHEN
1
;
Liang TANG
1
;
Xi-Kun SHEN
1
;
Yu-Yu HUANG
1
;
Ling FAN
2
;
Dong-Wei YU
2
Author Information
- Publication Type:Journal Article
- Keywords: angiogenesis; endothelial growth factor; H460; honokiol; lung cancer cells; NFκB
- From: Chinese Pharmacological Bulletin 2022;38(3):380-386
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of honokiol(HNK)on the angiogenesis of lung cancer cells H460 induced by the tumor inflammatory microenvironment and its possible mechanism.Methods CCK-8 was used to detect the effect of HNK on the proliferation of H460 cells and human umbilical vein endothelial cells(HUVECs); RT-PCR, Western blot, immunofluorescence were used to detect the expression of vascular endothelial growth factor(VEGF); Western blot was used to detect the signaling pathway protein p-IκBα, p-IKKα and NF-κB p65 protein expression.The wound healing test, transwell and tube formation tests were used to detect the inhibition ability of HNK on the migration and angiogenesis of HUVECs.Results HNK treated H460 cells for 24, 48, 72 h respectively, and inhibited the proliferation of H460 cells in a concentration-and time-dependent manner.HNK inhibited the survival of HUVEC cells in a concentration-dependent manner.HNK also reduced the protein and mRNA expression levels of VEGF in H460 cells.Subsequently, HNK concentration-dependently inhibited the phosphorylation of IκBα, NF-κB and IKKα in the NF-κB signaling pathway.Wound healing test, Transwell cell migration test and tube formation test showed that H460 conditioned medium treated with HNK had the ability to inhibit the migration and angiogenesis of HUVECs.Conclusions HNK reduces the viability and angiogenesis of human lung cancer cells by down-regulation of VEGF via the NF-κB pathway.
