The protective effect of essential oil from fructus alpiniae zerumbet on T2DM induced pancreatic injury in mice based on p62/Keapl/Nrf2 signaling pathway
- Author:
Hong YANG
1
;
Ting-Ting CHEN
1
;
Xiao MA
1
;
Ban ZHANG
1
;
Yue LI
1
;
Hong YANG
2
;
Xiao-Mei SONG
2
;
Xiang-Chun SHEN
2
;
Yong-Xin CHEN
3
;
Xiao-Mei SONG
3
;
Xiang-Chun SHEN
3
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; diabetes; EOFAZ; p62/ Keapl/Nrf2 signaling pathway; pancreas; pancreatic dysfuction
- From: Chinese Pharmacological Bulletin 2022;38(4):613-618
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effect of essential oil from Fructus Alpiniae Zerumbet (EOFAZ ) on type 2 diabetes-induced pancreatic injury in mice and its mechanism.Methods After C57 BL/6 mice fed with high-sugar and high-fat ( HFS) feed developed insulin resistance, streptozotocin ( STZ, 120 mg • kg 1 ) was injected intraperitoneal^ to establish type 2 diabetes model ( DM ) , then DM mice were randomly divided into: diabetes group ( DM ) , EOFAZ low-dose group (90 mg • kg 1 ) , EOFAZ high-dose group ( 180 mg • kg"1 ) , and metformin group (250 mg • kg 1 ).Healthy mice were randomly divided into : normal group and EOFAZ toxicity group ( 180 mg • kg"1).EOFAZ group and metformin group were given EOFAZ and metformin,while normal group and DM group were given an equal volume of normal saline once daily.After eight weeks of continuous intragastric administration, the mice were sacrificed, and the blood was collected to measure fasting blood glucose.HE staining was used to observe the pathological morphology of the pancreas; Western blot was used to analyze the expression of p62, Keleh-like epiehlorohvdrin-related protein 1 ( Keapl ), nuelear factor E2-related factor 2 ( Nrf2 ), and heme oxygenase- 1 ( HO-l ) , anti-apoptotic protein ( Bcl-2) and pro-apoptotic protein ( Bax).Immunohis- tochemistry was used to analyze the expression of Nr£2 in pancreatic tissue.Results Compared with DM group, after administration of EOFAZ , the pathological morphology of pancreas was significantly improved; Keapl , Bax protein expressions were significantly down-regulated and p62, Nrf2 , Ho-1 , Bcl-2 protein expressions were up-regulated ( P <0.05 ).Conclusions EOFAZ has a significant improvement effect on pancreatic injury induced by diabetes, and its effect may be related to the activation of p62/Keapl/Nrf2 to regulate apoptotic response.
