The protective effect of puerarin on MIN6 cell injury induced by palmitic acid
- Author:
Ming CHEN
1
;
Yu-Tong GAO
1
;
Xiao-Lin ZHANG
1
;
Ya-Ni WU
1
;
Xiu-Sha WEI
1
;
Hen-Bin HUANG
1
;
Guang-Yan MO
2
;
Xi YE
2
;
Xiao-Hui XU
3
;
Tao LIANG
4
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; diabetes; high fat; inflammation; MIN6 cells; puerarin
- From: Chinese Pharmacological Bulletin 2022;38(6):893-897
- CountryChina
- Language:Chinese
- Abstract: Aim To evaluate the effects of puerarin (PR) on pancreatic islet MIN6 cell injury and apopto- sis induced by palmitic acirl ( PA).Methods MIN6 cells pretreated with 2 h different concentrations of PR were then co-cultured with 120 (xmol • L"1 PA for 24 h to establish the cell injury and apoptosis model.MTT, LDH,MDA and GSH were used to determine the dam¬age of MIN6 cells.AOEB fluorescence staining was used to detect the apoptosis of MIN6 cells.Western blot was used to detect the expressions of inflammation- related protein NF-kB , apoptosis-related factors Bcl-2 and Bax.Results Compared with model group, cell viability and GSH activity of puerarin administration groups increased, LDH and MDA contents decreased.the protein expressions of p-NF-KB and Bax were down-regulated, and the protein expressions of Bcl-2 were up-regulated (P <0.05).Conclusions Puerar- in ean improve the function of pancreatic islet cells by inhibiting apoptosis and inflammation, and ameliorate pancreatic islet MIN6 cell injury and apoptosis induced by palmitic acid-induced, alleviate MIN6 cell injury in¬duced by inflammatory factors, which may be achieved by down-regulating the expression of p-NF-KB and Bax proteins,and up-regulating the expression of Bcl-2 pro¬tein.
