Soy isoflavones inhibit inflammation and apoptosis of hippocampal neurons induced by Ap, -42 through NF-kB p65 signaling pathway

Xiao-jie Shao; Wen-jie Guan; Zhan-mei Hong; Dong-lai Lyu; Xu-dong Zhang; Ming Chen; Fan Wang; Dong-lai Lyu

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Soy isoflavones inhibit inflammation and apoptosis of hippocampal neurons induced by Ap, -42 through NF-kB p65 signaling pathway

Author: Xiao-Jie SHAO ¹ ; Wen-Jie GUAN ¹ ; Zhan-Mei HONG ¹ ; Dong-Lai LYU ¹ ; Xu-Dong ZHANG ¹ ; Ming CHEN ² ; Fan WANG ² ; Dong-Lai LYU ³
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1. The 901 S/ Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Dept of Pharmacy
2. School of Basic Medical Sciences, Anhui Medical University, Dept of Pharmacy
3. Southivest Cancer Center, Army Medical University
Publication Type:Journal Article
Keywords: apoptosis; hippocampal neurons; neuroinflammation; NF- kB p65; p-amyloid 1-42; soy isoflavones
From: Chinese Pharmacological Bulletin 2022;38(6):874-879
CountryChina
Language:Chinese
Abstract: Aim To explore the effeet of soy isofla- vones (SI) on p-amyloid 1 -42 ( Ap, _42 ) -induced hippocampal neuroinflammation and neuronal apoptosis and the underlying mechanism.Methods The prima¬ry hippocampal neurons cultured in vitro were divided into control group (control), Ap,_42 treatment group f model) , SI low-dose group ( Sl-L, 10 mg • L 1 ) , and SI medium-dose group (SI-M, 20 mg • L_l ) and SI high-dose group (SI-H, 40 mg • L 1 ).The model group was treated with 30 (xmol • L"1 Ap, _42 for 48 h; the SI-L, SI-M and SI-H groups were treated with SI for 2 hours, and Ap,_42 was treated for 48 h; the con¬trol group was routinely cultured for 48 h.MTT method was used to detect the survival rate of hippocampal neurons; TUNEL staining was used to detect the apop¬tosis rate of hippocampal neurons; Western blot was used to detect COX-2, TNF-a, NF-kB p65 , P-NF-kB p65, Bcl-2 and caspase-3 protein expression levels.Results Compared with the control group, the surviv¬ al rate of hippocampal neurons was significantly re- duced (P <0.01) , and the apoptotie rate significantly increased (P<0.01).COX-2, TNF-a, p-NF-KB p65 , caspase-3 protein expressions markedly increased (P <0.05 or P <0.01 ) , and the expression of Bcl-2 protein significantly decreased in the model group ( P <0.01 ).Compared with the model group, the surviv¬al rate of hippocampal neurons, Bcl-2 protein in-creased, and the apoptotic rate, the expression of COX-2, TNF-a, p-NF-KB p65 , caspase-3 protein de¬creased (P < 0.05 or P < 0.01 ) in SI each dose group.Conclusion SI can reduce the hippocampal neuroinflammation and neuronal apoptosis induced by APi _42 by inhibiting the activation of NF-kB p65 signa¬ling pathway.