The biological activity of prodigiosin on adriamycin-resistant breast cancer cells in vitro

Han-cong Liu; Pei-bin Lin; Ya-er Zhang; Ya-er Wangjie; Wen-bin Liu; Xiao-bao Jin; Yan MA

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The biological activity of prodigiosin on adriamycin-resistant breast cancer cells in vitro

Author: Han-Cong LIU ¹ ; Pei-Bin LIN ¹ ; Ya-Er ZHANG ¹ ; Ya-Er WANGJIE ¹ ; Wen-Bin LIU ¹ ; Xiao-Bao JIN ¹ ; Yan MA ¹
Author Information

1. Guangdong Provincial Key Laboratory^ of Pharmaceutical Bioactive Substance, School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University
Publication Type:Journal Article
Keywords: adriamycin-resistant breast eaneer cells; apoptosis; cell cycle; Key words; mi¬gration; prodigiosin; proliferation
From: Chinese Pharmacological Bulletin 2022;38(6):865-873
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of prodigiosin on the proliferation, migration, cell cycle and apopto- sis of adriamycin-resistant breast cancer cell line MCF- 7/ADR.Methods CCK-8, colony formation assay, scratch test and Transwell were used to detect the pro¬liferation and migration of MCF-7/ADR after treatment with different concentrations of prodigiosin.How cy¬tometry and DAP1 staining were used to detect the cell cycle and apoptosis of MCF-7/ADR cell line after treatment with different concentrations of prodigiosin.Western blot was used to detect the effect of prodigiosin on the expression of caspase-3, Bax and Bcl-2 in MCF- 7/ADR cells.Results Prodigiosin inhibited the pro¬liferation and migration of MCF-7/ADR cell line in a concentration- and time-dependent manner ( P <0.05 ).In addition, prodigiosin enhanced the in¬hibitor}' effect of adriamycin on MCF-7/ADR cell line.Prodigiosin arrested MCF-7/ADR cell line cycle in the S phase and induced cell apoptosis in a time- and dose- dependent manner.The percentage of S phase cells treated with 2 mg • L 1 prodigiosin for 48 hours in¬creased to 35.3% , and the apoptotic rate was as high as 64.83% , which were statistically significant com¬pared with the control group ( P < 0.05 ).Prodigiosin could up-regulate the expression of apoptosis protein caspase-3 and Bax and inhibit the expression of Bcl-2 protein in MCF-7/ADK cell.Conclusions Prodigio¬sin can effectively inhibit the proliferation, migration and promote cell apoptosis and regulate cell cycle in adriamvcin-resistant breast cancer cell line MCF-7/ ADR.Prodigiosin can enhance the sensitivity of MCF- 7/ADR cell line to adriamycin.