Effect of Wenyang Huazhuo Tongluo formula on Sema3A/Nrpl signaling pathway in systemic sclerosis mouse model
- Author:
Ying-Li LI
1
;
Bo BIAN
1
;
Dong-Jiao LIU
1
;
Chen-Guang ZHAO
1
;
Yuan-Sen HAO
1
;
Li HAN
1
;
Hua BIAN
1
;
Ke-Lei GUO
1
;
Ying-Li LI
2
;
Li HAN
2
;
Hua BIAN
2
;
Ke-Lei GUO
2
Author Information
- Publication Type:Journal Article
- Keywords: Nrpl; Sema3A; systemic sclerosis; vasculopa-thy; VEGFA; Wenyang Huazhuo Tongluo formula
- From: Chinese Pharmacological Bulletin 2022;38(7):1088-1093
- CountryChina
- Language:Chinese
- Abstract: Aim To study the effeet of Wenyang Hua- Nrpl signaling pathway in systemic sclerosis ( SSc ) zhuo Tongluo formula ( WYHZTLF) on the Sema3A/ mouse model, and to explore its mechanism in the treatment of SSe.Methods The systemic sclerosis mouse model was constructed and divided into control group, model group, WYHZTLF low (21 g • kg 1 ) , medium (42 g • kg 1 ) , high (84 g • kg 1 ) dose group and the Sema3A inhibitor epigallocatechol gallate (EGCG) group.All groups were given intragastric administration for four weeks, and the control and model groups were treated with saline.Histopathology and dermal thickness were detected by HE, VEGFA pro-tein expression levels were detected by immunohisto- chemistry; the protein expression levels of Sema3A, Nrpl and VEGFA were determined by Western blot; Sema3A expression levels in mouse serum were detec-terl by ELISA.Results Comparer] with model group, WYHZTLF significantly alleviated the skin lesions in systemic sclerosis mouse model, significantly inhibited the dermal thickness, inhibited the protein expression levels of VEGFA, Sema3A and Nrpl , and reduced the expression levels of serum Sema3A.Conclusion WYHZTLF can improve the vascular injury of SSc, and its mechanism may be related to the inhibition of VEGFA and Sema3A/Nrpl signaling pathway.
