Role of mechanosensitive ion channel Piezol in mediating phenotypic changes of rat coronary arterial smooth muscle cells induced by high hydrostatic pressure

Sui-min LI; Xiao-yue Qin; Shu-zhen Chen; Chun-yu Deng; Sui-min LI; Xiao-yue Qin; Peng Zeng; Shu-zhen Chen; Su-juan Kuang; Hui Yang; Fang Rao; Chun-yu Deng; Peng Zeng; Chun-yu Deng

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Role of mechanosensitive ion channel Piezol in mediating phenotypic changes of rat coronary arterial smooth muscle cells induced by high hydrostatic pressure

Author: Sui-Min LI ¹ ; Xiao-Yue QIN ¹ ; Shu-Zhen CHEN ¹ ; Chun-Yu DENG ¹ ; Sui-Min LI ² ; Xiao-Yue QIN ² ; Peng ZENG ² ; Shu-Zhen CHEN ² ; Su-Juan KUANG ² ; Hui YANG ² ; Fang RAO ² ; Chun-Yu DENG ² ; Peng ZENG ³ ; Chun-Yu DENG ³
Author Information

1. School of Biological Science and Engineering South China University of Technology
2. Medical Research Center of Guangdong Provincial People' S Hospital, Guangdong Provincial Key Laboratory of Clinical Pharmacology, Guangdong Academy of Medical Sciences
3. School of Medicine, South China University of Technology
Publication Type:Journal Article
Keywords: contractile pheno- type; coronary arterial smooth muscle cells; high hydrostatic pressure; phenotypic change; Piezol; synthetic phenotype
From: Chinese Pharmacological Bulletin 2022;38(8):1202-1208
CountryChina
Language:Chinese
Abstract: Aim To investigate the mechanism of Pi- ezol in the phenotypic changes of rat coronary arterial smooth muscle cells ( CASMCs) induced by high hydrostatic pressure.Methods CASMCs were isolated from Wistar rats and stimulated for 24 h at 0, 120 and 180 mmHg, respectively.The expressions of Piezol , contractile phenotvpe-related proteins including Cavl.2 ,SM-MHC ,cx-SMA and synthetic phenotvpe-re- lated proteins including OPN , MMP-2, Coll al were detected by Western blot.The effect of calcium influx mediated by Piezol was detected by Laser confocal mi- j j croscopy.CASMCs were treated with Piezol agonist Yodal , inhibitor GsMTx4 and Piezol-siHNA , respectively and the expressions of contractile phenotvpe and synthetic phenotvpe-related proteins were detected by Western blot.Results Compared with control ( 0 mmHg) , the expressions of Piezol , OPN, MMP-2 and Collal increased, but the expressions of Cavl.2,SM- MHC and cx-SMA decreased in 120 mmHg as well as 180 mmHg group.After stimulated by 180 mmHg high pressure, Piezol-mediated calcium influx was stronger than that in 0 mmHg group, hut decreased after Piezol knockdown.Treated with Yodal at 0 mmHg, the expression of contractile phenotvpe-related protein decreased while the expression of synthetic phenotvpe-re- lated protein increased compared with DMSO group..\Jfter using GsMTx4 to inhibit or siRNA to knockdown Piezol at 180 mmHg,the expression of contractile phe- notvpe-related protein increased and the expression of synthetic phenotype-related protein decreased compared with the control group.Conclusion Piezol promotes the transition from contractile phenotvpe to syn-thetic phenotvpe of CASMCs induced by high hydrostatic pressure.