Transitional Zone of the Corneal Endothelium: Flat Preparation and Ultrastructural Findings.
- Author:
O Sub KOO
1
;
Myung Kyoo KO
Author Information
1. Department of Ophthalmology, Hanyang University College of Medicine, Seoul, Korea. fovea@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Corneal endothelial cell;
Flat preparation;
Trabecular meshwork;
Transitional zone;
Ultrastructure
- MeSH:
Endothelial Cells;
Endothelium, Corneal*;
Microscopy;
Microscopy, Electron;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2007;48(5):691-698
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the morphological characteristics of the transitional zone between the corneal endothelium and the trabecular meshwork by flat preparation and electron microscopy. METHODS: The materials comprised 12 eyes examined by the flat preparation and 7 eyes by the electron microscopy. The specimens were derived from the transitional tissue between the corneal endothelium and the trabecular meshwork. The specimens in the flat preparation were stained with hematoxylin-eosin and examined by light microscopy. The specimens for scanning electronic microscopy (SEM) and in transmission electronic microscopy (TEM) were examined through routine processes. RESULTS: In the specimens examined by the flat preparation, unlike peripheral corneal endothelial cells, the endothelial cell nuclei in the transitional zone were overlapped and morphologically oval. On SEM, unlike typical hexagonality and tight interdigitation of corneal endothelial cells, the endothelial cells in the transitional zone were partially successive, spaced intercellularly, and morphologically irregular. On TEM, the endothelial cells in the transitional zone were partially successive. CONCLUSIONS: The loss of cell-cell contact of endothelial cells in the transitional zone may lead to the potential proliferation capacity of endothelial cells in the transitional zone under specific conditions. Therefore, further studies on the proliferation capacity of endothelial cells in the transitional zone are needed together with more research on cell biology.
