Diallyl disulfide downregulates XIAP through miR-7 to inhibit proliferation, migration and invasion of human gastric cancer SGC7901 cells
- Author:
Fang1 LIU
1
;
Zhi-Min LI
1
;
Zhen-Hua CAO
1
;
Hui HE
1
;
Qi SU
1
;
Bo SU
1
;
Hui HE
2
;
Bo SU
3
Author Information
- Publication Type:Journal Article
- Keywords: diallyl disulfide; gastric cancer; invasion; migration; miR-7; proliferation; X-linked inhibitor of apoptosis protein
- From: Chinese Pharmacological Bulletin 2022;38(8):1164-1169
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate whether diallyl disul¬fide ( DADS) downregulates X-linked inhibitor of ap- optosis protein ( XIAP) through miR-7 , inhibiting the proliferation, migration anrl invasion of gastric eaneer SGC7901 eel Is.Methods Gastric eaneer SGC7901 eell line overexpressing XIAP was established.qRT- PCR and Western blot were used to deteet the effeet of DADS on XIAP expression in overexpressed eells.CCK-8 and plate elone formation assay were used to analyze the effeet of DADS on the proliferation of XIAP overexpressing eells.Wound healing and Transwell ex¬periments were employed to analyze the effeets of DADS on the migration and invasion of XIAP overex¬pressing eells.qRT-PCR was used to deteet the effeet of DADS on the expression of miR-7.Negative regula¬ tion of miR-7 on XIAP was verified by using dual lucif- erase reporter gene assay, qRT-PCR ,anrl Western blot.Results XIAP overexpression enhanced the prolifera¬tion , migration and invasion of SGC7901 cells.DADS downregulated XIAP and inhibited the proliferation, mi¬gration and invasion of XIAP overexpressing cells.DADS upregulated miR-7 expression.miR-7 bound and regulated XIAP 3 'UTR directly.Overexpression of miR-7 decreased XIAP expression,while knockdown of miR-7 increased XIAP expression.Conclusion DADS downregulates XIAP through miR-7 to inhibit SGC7901 cell proliferation, migration and invasion.
