Paeonol inhibits rheumatoid arthritis through formyl peptide receptors
- Author:
Xiao-Si YANG
1
;
Shu-Yan SHENG
1
;
Yu-Bao SHAO
2
;
Xiao-Yu CHEN
2
;
Li WEN-HAO
3
;
Meng-Meng CHEN
3
;
Jia-Hao WANG
3
;
Ze-Rui GONG
4
Author Information
- Publication Type:Journal Article
- Keywords: bioinformatics; fibroblasts; formyl peptide receptor 2; inflammation; paeonol; rheumatoid arthritis
- From: Chinese Pharmacological Bulletin 2022;38(9):1320-1327
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the pharmacological effects of paeonol as a formyl peptide receptor activator on rheumatoid arthritis (HA).Methods The target rlataset was obtained from the high throughput Gene Expression Database ( GEO) , and multiple data sets were combined by USING R language to explore three groups of macrophage differentially expressed genes ( DEGs) in untreated,lipopolysaccharide (LPS) treat¬ment and paeonol and LPS treatment, and their enrich-ment pathway was analyzed.Protein-protein interaction (PPI) networks were constructed in the STRING data¬base anrl visualized in Cytoscape software.The inhibi¬tor}' effect of Hub gene formyl peptide receptor ( FPR) on RA inflammation was validated by TNF-cx stimula¬tion of fibroblast synovial cells ( FLS).Results Through bioinformatics analysis, 169 differential genes ( DEGs) related to inflammation and 275 DEGs related to the mechanism of paeonol action were obtained.Combined analysis of the two groups of DEGs showed that FPR played a key role in the anti-inflammatory mechanism of paeonol.Further studies on the mecha¬nism of paeonol showed that paeonol activated FPR, and the inhibitory effect of paeonol on FLS inflamma¬tion was rescued by TRP-ARg-TRP-TRP-TRP-TRP- TRP-TRP-NH2 (WRW4).Conclusion Paeonol can inhibit the inflammatory development of RA through the FPR pathway.
