Mechanism of Rosa roxburghii Tratt in treatment of hyperlipidemia based on network pharmacology and molecular docking technology

Jia-cheng JL; Min-yan Yuan; Li Tang; Peng-jiao Wang; Min Zhang; Xiu-li Gao; Shuo Zhang; Min Zhang; Xiu-li Gao; Xiu-li Gao

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Mechanism of Rosa roxburghii Tratt in treatment of hyperlipidemia based on network pharmacology and molecular docking technology

Author: Jia-Cheng JL ¹ ; Min-Yan YUAN ¹ ; Li TANG ¹ ; Peng-Jiao WANG ¹ ; Min ZHANG ¹ ; Xiu-Li GAO ¹ ; Shuo ZHANG ² ; Min ZHANG ³ ; Xiu-Li GAO ³ ; Xiu-Li GAO ⁴
Author Information

1. State Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University
2. Experimental Animal Center of Guizhou Medical University
3. Center of Microbiology and Biochemical Pharmaceutical Engineering, Guizhou Medical University
4. Guizhou Provincial Engineering Research Center of Food Nutrition and Health, Guizhou Medical University
Publication Type:Journal Article
Keywords: animal experiment; att; hyperlipidemia; mechanism; molecular docking; network pharmacology
From: Chinese Pharmacological Bulletin 2022;38(10):1572-1578
CountryChina
Language:Chinese
Abstract: Aim To explore the ameliorative effects of Rosa roxburghii Tratt on hyperlipidemia and investigate the underlying mechanism by using experimental validation and network pharmacology.Methods The therapeutic effect of Rosa roxburghii Tratt on hyperlipidemia was investigated by constructing a hyperlipidemic rat model and measuring the serum lipid index and liver pathological changes.The literature search method was used to obtain active ingredients and targets of Rosa roxburghii Tratt,the target gene was collected from GeneCards,OMIM,DrugBank database,then generated herbal-active ingredient-potential target networks and protein-protein interactions(PPI)networks.The target GO function enrichment analysis and KEGG pathway enrichment analysis were analyzed by DAVID software.Molecular docking was carried out using AutoDock.Results Rosa roxburghii Tratt could significantly improve dyslipidemia and liver pathological damage in hyperlipidemic rats.Network pharmacology results showed that RXRA,AKT1,ESR1,PIK3R1 were key targets of Rosa roxburghii Tratt to lower blood lipids.Molecular docking showed that Roxburic acid and α-linolenic acid had good binding to RXRA,AKT1,ESR1,and PIK3R1.Conclusions Combined with pharmacodynamic experiments and network pharmacology-molecular docking,the therapeutic effect and possible mechanism of action of Rosa roxburghii Tratt on hyperlipidemia are preliminarily explored,which provides a certain basis for the in-depth study of pharmacodynamic substance basis,mechanism of action and clinical application.