Prevention and inhibition of nasopharyngeal carcinoma growth by attenuated salmonella SGN1
- Author:
Yun-Hao LAI
1
;
Ting-Qi HUANG
1
;
Shi LIU
1
;
Yue-Rong PENG
1
;
Fang-Hong LI
1
;
Zheng-Gang ZHAO
1
;
Su-Jin ZHOU
1
;
Zi-Jian ZHAO
1
;
Qi-Ting TAN
2
;
Jia-Luo MAI
3
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; attenuated salmonella; cell cyle; methioninase; nasopharyngeal carcinoma; SGN1
- From: Chinese Pharmacological Bulletin 2023;39(10):1867-1873
- CountryChina
- Language:Chinese
- Abstract: Aim To study the inhibitory effect of attenuated salmonella SGN1, overexpressing methioninase, on nasopharyngeal carcinoma (NPC) and the underlying mechanism. Methods The cell proliferation, cell cycle, cell apoptosis, clony formation and migration a-bility of 5-8F, HNE-2, CNE-2 cells were measured u-sing flow cytometry assay, clone formation assay, and wound assay after the methionine restriction treatment. 5-8F, HNE-2, CNE-2 cells were infected with SGN1 at the multiplicity of infection (MOI) of 1: 100 for 5 hours, followed with the measurement of cell growth. A xenograft model was constructed by subcutaneous injection of 5-8F cells in mice to observe the inhibitory effect of SGN1 on nasopharyngeal carcinoma. Results Compared with the control group, methionine restriction significantly inhibited the proliferation, migration ability, and clone formation of nasopharyngeal carcinoma cells and blocked the G
