Study on role of HDAC5 in P-gp expression in rats in high-altitude low oxygen environment and its effect on phenytoin sodium pharmacokinetics
- Author:
Yi-Lan ZHAO
1
;
Hong-Fang MU
1
;
Xiao-Jing ZHANG
1
;
Lin LUO
1
;
Jia-Xin HE
1
;
An-Peng ZHAO
1
;
Rong WANG
1
;
Yi-Lan ZHAO
2
;
Lin LUO
2
;
Shi-Ian FENG
2
;
Jia-Xin HE
2
;
Yi-Lan ZHAO
3
;
Hong-Fang MU
3
;
Xiao-Jing ZHANG
3
;
Lin LUO
3
;
Jia-Xin HE
3
;
An-Peng ZHAO
3
;
Rong WANG
3
Author Information
- Publication Type:Journal Article
- Keywords: epigenetics; HDAC5; high-altitude low oxygen; P-gp; pharmacokinetics; phenytoin sodium
- From: Chinese Pharmacological Bulletin 2023;39(9):1740-1745
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the important role of HDAC5 in P-gp expression in rats in high-altitude low oxygen environment and its effect on phenytoin sodium pharmacokinetics. Methods Wistar rats were transported to Batang, Yushu, Qinghai, at an altitude of 4010 m, with 6 rats in each group, divided into 1 d and 3 d groups. Different groups were given phenytoin, phenytoin combined with hypericin, and phenytoin combined with verapamil. Plasma and liver tissues were collected at different time after taking the drug in the plateau area. The concentration of phenytoin sodium in plasma was determined by UFLC-MS method. Changes in protein expression were detected by Western blot. Results The results of UFLC-MS showed that the AUC
