Research progress on hypoxia/reoxygenation model of nerve cells in vitro
- Author:
Man-Man WANG
1
;
Xue-Qin FU
1
;
Xu-Huan ZOU
1
;
Wei-Wei WANG
1
;
Zhen LEI
2
;
Yong ZHANG
3
;
Bao-Qi WANG
4
;
Rui LAN
4
Author Information
- Publication Type:Journal Article
- Keywords: cell model; construction method; hypoxia reoxygenation; ischemia reperfusion; model characteristics; nerve cells
- From: Chinese Pharmacological Bulletin 2023;39(5):823-828
- CountryChina
- Language:Chinese
- Abstract: Ischemic stroke is the second leading cause of human death and the third reason of disability. Meanwhile, the incidence is rising year after year worldwide. Ischemic stroke could cause ischemia-reperfusion injury after blood recanalization treat-ment, but the mechanism of ischemia-reperfusion injury is still not very clear, so it is necessary to build a preclinical model with specific characteristics. Up to now, animal experiments have been still complicated, and the culture of brain slices has some limitations. The cell model in vitro has become a simplified and valuable tool widely used by researchers. The paper systematically summarizes the common type of nerve cells, and further analyzes establishment methods and principle, relevant research progress on the in vitro model of ischemia-reperfusion, in order to provide reference for rationally selecting hypoxia and reoxygenation model for basic research on cerebral ischemia and reperfusion and drug screening.
