Mechanosensitive ion channel Piezo1 induces senescence of atrial fibroblasts by activating β-catenin
- Author:
Xing-Dong YE
1
;
Xue-Shan LUO
1
;
Qiao-Qiao LI
1
;
Jin-Tao HE
1
;
Yu-Wen XU
1
;
Hui YANG
1
;
Chun-Yu DENG
1
;
Su-Juan KUANG
1
;
Meng-Zhen ZHANG
1
;
Shu-Lin WU
1
;
Fang RAO
1
;
Yu-Mei XUE
1
Author Information
- Publication Type:Journal Article
- Keywords: atrial fibrillation; atrial fibroblast; Piezo1; senescence; structural remodeling; β-catenin
- From: Chinese Pharmacological Bulletin 2023;39(7):1234-1241
- CountryChina
- Language:Chinese
- Abstract: Aim To observe whether the mechanosensitive ion channel Piezo1 was involved in the senescence of atrial fibroblasts by activating β-catenin based on our previous study which found marked increase of Piezo1 mRNA in senescent atrial fibroblasts. Methods Primary mouse atrial fibroblasts (MAFs) were isolated from male C57BL/6 mice (3-4 weeks) by enzyme digestion, and tert-butyl hydroperoxide (TBHP) was used to induce the senescence of cells. The ratio of senescent cells was detected by senescence-associated β-galactosidase (SA-β-Gal) staining. The protein levels of Piezo1, β-catenin/p-β-catenin, senescence-associated proteins p53 and p21 in the cells treated with TBHP (100 μmol · L
