Triapine induces ferroptosis via ROS/GSH/GPX4 axis in A549 cells

Author: Peng-Fei ZHANG ¹ ; Dong HUA ¹ ; Li-Hua ZHANG ² ; Dong HUA ³
Author Information

1. School of Life Sciences and Health Engineering, Jiangnan University
2. Institute of Gastrointestinal Oncology, Medical College of Xiamen University
3. Dept of Oncology, Wuxi People's Hospital of Nanjing Medical University
Publication Type:Journal Article
Keywords: ferroptosis; GPX4; lipid peroxides; lung cancer; ROS; Triapine
From: Chinese Pharmacological Bulletin 2023;39(5):833-838
CountryChina
Language:Chinese
Abstract: Aim To investigate the induction of ferroptosis of Triapine in non-small cell lung cancer cells A549, and its mechanism. Methods The effects of Triapine on the proliferation of A549 cells were assessed by MTT assay and colony formation assay; the effect of intracellular ROS levels of Triapine treated A549 cells was studied by DCFH-DA probe; the intracellular glutathione (GSH) and lipid peroxides (LPO) levels of A549 cells were detected by the kits after treating with Triapine; the effects of Triapine on the expression of glutathione peroxidase 4 (GPX4) in A549 cells were analyzed by Western blot; the changes of GPX4 level and cell viability were evaluated for the cells intervened with ROS inhibitor. Results Triapine could inhibit the proliferation of A549 cells, and the IC