Research on ability of tetramethylpyrazine on promoting neurogenesis after OGD injury based on neural stem cell microenvironment

Author: Wan-Qing XING ¹ ; Xiao-Xiong SONG ¹ ; Xue-Qing WANG ¹ ; Jin-Fu LU ¹ ; Gao-Hong LYU ¹ ; Bin YU ¹ ; Ming RUAN ²
Author Information

1. Jiangsu Key Lab for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine
2. School of Food Science, Nanjing Xiaozhuang University
Publication Type:Journal Article
Keywords: astrocytes; brain microvascular endothelial cells; meural stem cells; microenvironment of neural stem cells; neurogenesis; tetramethylpyrazine
From: Chinese Pharmacological Bulletin 2023;39(4):731-738
CountryChina
Language:Chinese
Abstract: Aim To study the ability of tetramethylpyrazine (TMP) on promoting neurogenesis in neural stem cell microenvironment after oxygen-glucose deprivation (OGD) injury in vitro. Methods Neural stem cells (NSCs), astrocytes (ACs) and cerebral microvascular endothelial cells (BMECs) were respectively extracted and separated to establish a co-culture system. The OGD modeling conditions were optimized by NSCs activity, and the concentration of TMP was optimized by Nissl staining. Then CCK-8 and Nestin