Mechanism of grape seed proanthocyanidin oligomers inhibiting polarization of type Al astrocytes

Qing Wang; Zhi-chao Yang; Yi-wei Dong; Shu-wen Yuan; Yan-qing LI; Li-juan Song; Cun-gen MA; Jian-jun Huang; Cun-gen MA

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Mechanism of grape seed proanthocyanidin oligomers inhibiting polarization of type Al astrocytes

Author: Qing WANG ¹ ; Zhi-Chao YANG ¹ ; Yi-Wei DONG ¹ ; Shu-Wen YUAN ¹ ; Yan-Qing LI ¹ ; Li-Juan SONG ¹ ; Cun-Gen MA ¹ ; Jian-Jun HUANG ² ; Cun-Gen MA ³
Author Information

1. Research Center of Neurobiology, The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine
2. Dept of Neurosurgery, Sinopharm Tongmei General Hospital, Key Laboratory of Neurological Disease Prevention and Control of Shanxi Health Commission
3. Institute of Brain Science, Medical School, Shanxi Datong University
Publication Type:Journal Article
Keywords: A1; astrocytes; cytokines; GSPs; JNK; neurotrophic factors
From: Chinese Pharmacological Bulletin 2023;39(1):77-83
CountryChina
Language:Chinese
Abstract: Aim To explore the mechanism of grape seed proanthocyanidins (GSPs) targeting astrocytes (AS), so as to regulate the phenotype and function of AS and maximize their neuroprotective effect. Methods The effects of GSPs on the phenotype, secretion of pro-inflammatory factors and neurotrophic factors of Al AS induced by TNF-α, IL-1α and Clq were investigated by RT-PCR, Elisa and Western blot in vitro. And JNK phosphorylation was determined using Western blot. Results GSPs significantly reduced the expression of C3d and Clq of Al AS markers and inhibited the phosphorylation of JNK. Moreover, compared with the model group, GSPs could significantly inhibit the release of pro-inflammatory cytokines IL-6, IL-1 α, IL-17 and H