Daidzein affects proliferation and apoptosis in non-small cell lung cancer cells:role of p53 signaling pathway
- Author:
Ting LUO
1
;
Shi-Qun YU
1
;
Jin OU-YANG
1
;
Ting LUO
2
;
Shi-Qun YU
2
;
Jin OU-YANG
2
;
Deng-Gang FU
2
;
Xiao-Wei XIONG
2
;
Shao-Xin HUANG
2
;
Xin WANG
2
;
Xiao-Bo WANG
3
;
You PENG
4
;
Cheng-Quan CHENG
5
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; daidzein; non-small cell lung cancer cells; p53 signaling pathway; proliferation; soy isoflavone
- From: Chinese Pharmacological Bulletin 2023;39(3):431-438
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of daidzeinDD on the proliferation and apoptosis of non-small cell lung cancer cells,with a focus on the possible role of the p53 signaling pathway in this regard. Methods CCK-8 method and flow cytometry were used to detect the effects of soy isoflavone crude extract and DD on the viability and apoptosis of HELF and H1299 cells. Gene microarray was used to detect the changes in gene expression after treatment of H1299 cells with DD. GSEA and differential analysis were used to screen the major pathways and key genes. RT-qPCR and Western blot were performed to verify the differences in mRNA and protein expression of key genesp53 and CASP9 in the major pathways. After p53 inhibitor Pifithrin-α inhibited the expression of p53,the effect of DD on p53 mRNA and protein expression levels was examined,and the proliferative effect on H1299 cells was observed. Results Soy isoflavone crude extract and DD promoted proliferation and inhibited apoptosis of normal lung cells and inhibited proliferation and promoted apoptosis of lung cancer cells. p53 signaling pathway was significantly enriched in the DD-treated groupNES=1.78,P=0.000,and the expressions of p53 and CASP9 genes were found to be significantly up-regulated in the treated group. Compared with the control group,mRNA expression of CASP9 and p53 significantly increased in both HELF and H1299 cells treated with DDP<0.05,and p53 protein expression also increased in HELF cellsP<0.05. After inhibition of p53 expression,DD significantly increased the mRNA expression of p53 in H1299 and HELF cellsP<0.05 and also markedly increased the expression of p53 protein in H1299 cellsP<0.05,and it was observed that DD inhibited the proliferation of lung cancer cells. Conclusions DD inhibits the proliferation and promotes the apoptosis of lung cancer H1299 cells,and the mechanism mainly involves the p53 signaling pathway.
