Mechanism study of OVA-induced allergic asthma in mice based on lipid metabolomics
- Author:
Xiao-Juan RONG
1
;
Yu-Tong KANG
1
;
Lei XU
1
;
Ling-Fei KONG
2
;
Tuo QIN
2
;
Cai TIE
2
Author Information
- Publication Type:Journal Article
- Keywords: allergic asthma; eicosanoids; linoleic acids; lipid mediators; mechanism research; metabolomics
- From: Chinese Pharmacological Bulletin 2023;39(3):477-483
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the mechanism and search for potential biomarkers of ovalbumin ( OVA ) -induced asthma in mice base on lipidomics. Methods A BALB/c mouse model of asthma was prepared by OVA. TNF-α, IL-4, IL-10, IFN-γ levels in BALF and IgE level in serum were measured by ELISA. The inflammatory changes in mouse lung tissue were observed using HE staining. Lipid mediators ( LMs) in lung tissue and serum were quantified with UPLC-MS/ MS strategy. Results IgE level in serum and TNF-α, IFN-γ levels in BALF were higher (P <0.05) of asthmatic mice.Typical inflammatory manifestations were seen in lung tissue of asthmatic mice. A total of 57 lipid mediators were quantified with UPLC-MRM. LMs metabolic profiles differed significantly in serum and lung tissue between asthmatic and normal mice, 17 significantly different LMs were found in lung tissue and 6 LMs were found in serum, and the differential metabolites were produced through the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 oxidase (P450) metabolic pathways. Conclusions OVA-induced allergic asthma can cause disorder of lip-id mediators, LMs and cytokines are involved in the occurrence and development of asthma. The differential LMs have potential research value as biomarkers for the development of allergic asthma.
