Effects of Zishen Huoxue Prescription on OGD/R-induced mitophagy in hippocampal neurons
- Author:
Tong-He LIU
1
;
Jia-Yi SHI
1
;
Bo-Jing ZHANG
1
;
Qian-Rou MA
1
;
Run-Cheng ZHANG
1
;
Xiu-Li ZHANG
1
;
Da-Hua WU
2
;
Zi-Ting ZHAO
2
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; medicated serum; mitophagy; OGD/R; primary hippocampal neurons; Zishen Huoxue Prescription
- From: Chinese Pharmacological Bulletin 2023;39(6):1189-1194
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the protective effect of Zishen Huoxue Prescription on OGD/R-induced primary hippocampal neuron damage in rats and the possible mechanism. Methods After the isolated primary hippocampal neurons were identified by immunofluorescence, OGD/R induced neuronal damage, and the changes of autophagic flux at different re-oxygenation time were observed by confocal laser scanning microscopy. After OGD/R-induced primary hippocampal neurons were intervened with serum containing Zishen Huoxue Prescription, cell viability was detected by CCK-8, cell apoptosis was detected by flow cytometry, autophagosomes were detected by transmission electron microscopy, and autophagy-related protein expressions were detected by Western blot. Results 10% Zishen Huoxue Prescription-containing serum could significantly improve cell viability and reduce the proportion of cell apoptosis, increase the number of autophagosomes in neurons, and up-regulate the expression of autophagy-related protein PINK1, Parkin, and pATG16L1. Conclusions Zishen Huoxue Prescription can effectively resist OGD/R-induced apoptosis of primary hippocampal neurons in rats, and its effect may be related to the regulation of PINK1-Parkin pathway to promote mitophagy.
