Effect of Rubus irritans Focke extract on differentiation of 3T3-L1 preadipocytes

Author: Yue LIU ¹ ; Song YU ¹ ; Yong-Xia HAN ¹ ; Li TONG ¹ ; Fang YANG ¹ ; Fang-Fang TIE ² ; Li TONG ³ ; Fang YANG ³
Author Information

1. Medical College, Qinghai University
2. Northwest Institute of Plateau Biology, Chinese Academy of Sciences
3. Qinghai Provincial Key Laboratory of Traditional Chinese Medicine Research for Glucolipid Metabolic Diseases
Publication Type:Journal Article
Keywords: 3T3-L1 adipocytes; AMPK; lipid accumulation; lipid metabolisim; PPAR-y-; Rubm irritans Focke extract
From: Chinese Pharmacological Bulletin 2023;39(6):1090-1096
CountryChina
Language:Chinese
Abstract: Aim To study the effect of Rubus irritans Focke extract on adipogenic differentiation of 3T3-L1 preadipocytes, and to further explore the potential mechanism of Rubus irritans Focke on adipocyte metabolism, so as to provide a new basis for the prevention and treatment of obesity. Methods Rubus irritans Focke extract was separated and prepared by MCI medium pressure chromatographic column. MTT method was used to detect cell proliferation, and oil red 0 staining and kit test was used to to detect the level of lipid accumulation. Western blot was employed to detect the expressions of peroxisome proliferator-activated receptor ¦y (PPAR7), CCAAT enhancer binding protein a (C/ EBPa), adenosine 5'-monophosphate activated protein kinase (AMPK) and phosphorylated AMPK (p-AMPK) protein. Results When the concentration of Rubus irritans Focke extract was less than 100 mg •L