Age-dependent differences in myelin basic protein expression in the hippocampus of young, adult and aged gerbils.
10.5625/lar.2017.33.3.237
- Author:
Ji Hyeon AHN
1
;
Tae Kyeong LEE
;
Joon Ha PARK
;
Jeong Hwi CHO
;
In Hye KIM
;
Jae Chul LEE
;
Seongkweon HONG
;
Yong Hwan JEON
;
Il Jun KANG
;
Young Joo LEE
;
Moo Ho WON
;
Choong Hyun LEE
Author Information
1. Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Korea.
- Publication Type:Original Article
- Keywords:
Myelin basic protein;
aging;
hippocampus;
gerbil
- MeSH:
Adult*;
Aging;
Blotting, Western;
CA1 Region, Hippocampal;
Gerbillinae*;
Hippocampus*;
Humans;
Immunohistochemistry;
Myelin Basic Protein*;
Myelin Sheath*;
Perforant Pathway
- From:Laboratory Animal Research
2017;33(3):237-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
Myelin degeneration is one of the characteristics of aging and degenerative diseases. This study investigated age-related alterations in expression of myelin basic protein (MBP) in the hippocampal subregions (dentate gyrus, CA2/3 and CA1 areas) of gerbils of various ages; young (1 month), adult (6 months) and aged (24 months), using western blot and immunohistochemistry. Western blot results showed tendencies of age-related reductions of MBP levels. MBP immunoreactivity was significantly decreased with age in synaptic sites of trisynaptic loops, perforant paths, mossy fibers, and Schaffer collaterals. In particular, MBP immunoreactive fibers in the dentate molecular cell layer (perforant path) was significantly reduced in adult and aged subjects. In addition, MBP immunoreactive mossy fibers in the dentate polymorphic layer and in the CA3 striatum radiatum was significantly decreased in the aged group. Furthermore, we observed similar age-related alterations in the CA1 stratum radiatum (Schaffer collaterals). However, the density of MBP immunoreactive fibers in the dentate granular cell layer and CA stratum pyramidale was decreased with aging. These findings indicate that expression of MBP is age-dependent and tissue specific according to hippocampal layers.
