Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells.
10.5625/lar.2017.33.3.244
- Author:
Phil Ok KOH
1
Author Information
1. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
α-synuclein;
cerebral ischemia;
hippocampal-derived cell line;
MCAO
- MeSH:
Blotting, Western;
Brain Ischemia;
Brain*;
Cell Death;
Cell Line;
Cerebral Cortex;
Glutamic Acid;
Humans;
Infarction;
Infarction, Middle Cerebral Artery;
Male;
Nervous System Diseases;
Neurodegenerative Diseases;
Neurons;
Neuroprotective Agents;
Oxidative Stress;
Rats, Sprague-Dawley
- From:Laboratory Animal Research
2017;33(3):244-250
- CountryRepublic of Korea
- Language:English
-
Abstract:
α-Synuclein is abundantly expressed in neuronal tissue, plays an essential role in the pathogenesis of neurodegenerative disorders, and exerts a neuroprotective effect against oxidative stress. Cerebral ischemia causes severe neurological disorders and neuronal dysfunction. In this study, we examined α-synuclein expression in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury and neuronal cells damaged by glutamate treatment. MCAO surgical operation was performed on male Sprague-Dawley rats, and brain samples were isolated 24 hours after MCAO. We confirmed neurological behavior deficit, infarction area, and histopathological changes following MCAO injury. A proteomic approach and Western blot analysis demonstrated a decrease in α-synuclein in the cerebral cortices after MCAO injury. Moreover, glutamate treatment induced neuronal cell death and decreased α-synuclein expression in a hippocampal-derived cell line in a dose-dependent manner. It is known that α-synuclein regulates neuronal survival, and low levels of α-synuclein expression result in cytotoxicity. Thus, these results suggest that cerebral ischemic injury leads to a reduction in α-synuclein and consequently causes serious brain damage.
