Protein phosphorylation involved in psychedelic-induced head-twitch response in rats

Author: Shao-Wen WANG ¹ ; Ya-Nan ZHOU ¹ ; Shao-Wen WANG ² ; Ya-Nan ZHOU ² ; Yi SUN ² ; Rui-Bin SU ²
Author Information

1. Nanjing University of Chinese Medicine
2. State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences
Publication Type:Journal Article
Keywords: acetylation; animal model; head-twitch response; phosphorylation; prefrontal cortex; protein post-translational modifications; psychedelics; ubiquitination
From: Chinese Pharmacological Bulletin 2023;39(10):1899-1904
CountryChina
Language:Chinese
Abstract: Aim To investigate the potential protein post-translational modifications of psychedelic-induced Head-twith response and underling mechanism. Methods Psychedelics LSD, DOM, or Psilocin was administered to rats by intraperitoneal injection to induce head-twitch response, then the most effective dosage was identified to create animal models of head-twitch behavior. Western blot was performed in detecting the protein phosphorylation, acetylation, and ubiquitination in prefrontal cortex of SD rats after 10 min or 30 min injection. Results LSD (0.025 mg • kg~, i. p.), DOM (3 mg•kg