Lycium barbarian seed oil activates Nrf2/ARE pathway to reduce oxidative damage in testis of subacute aging rats
- Author:
Rui-Ying TIAN
1
;
Wen-Xin MA
1
;
Zi-Yu LIU
1
;
Hui-Ming MA
1
;
Sha-Sha XING
2
;
Na HU
2
;
Chang LIU
2
;
Biao MA
3
;
Jia-Yang LI
3
;
Hu-Jun LIU
3
;
Chang-Cai BAI
4
;
Dong-Mei CHEN
5
Author Information
- Publication Type:Journal Article
- Keywords: Lycium barbarum seed oil; Nrf2/ARE signaling pathway; oxidative stress; reproductive hormone; subacute senescence; testicle
- From: Chinese Pharmacological Bulletin 2024;40(3):490-498
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effects of Lycium berry seed oil on Nrf2/ARE pathway and oxidative damage in testis of subacute aging rats. Methods Fifty out of 60 male SD rats, aged 8 weeks, were subcutaneously injected with 125 mg • kg"D-galactosidase in the neck for 8 weeks to establish a subacute senescent rat model. The presence of senescent cells was observed using P-galactosidase ((3-gal), while testicular morphology was examined using HE staining. Serum levels of testosterone (testosterone, T), follicle-stimulating hormone ( follicle stimulating hormone, FSH ) , luteinizing hormone ( luteinizing hormone, LH ) , superoxide dis-mutase ( superoxide dismutase, SOD ) , glutathione ( glutathione, GSH) and malondialdehyde ( malondial-dehyde, MDA) were measured through ELISA, and the expressions of factors related to aging, oxidative damage, and the Nrf2/ARE pathway were assessed via immunohistochemical analysis and Western blotting. Results After successfully identifying the model, the morphology of the testis was improved and the intervention of Lycium seed oil led to a down-regulation in the expression of [3-gal and -yH2AX. The serum levels of SOD, GSH, T, and FSH increased while MDA and LH decreased (P 0. 05) . Additionally, there was an up-regulated expression of Nrf2, GCLC, NQOl, and SOD2 proteins in testicular tissue ( P 0. 05 ) and nuclear expression of Nrf2 in sertoli cells. Conclusion Lycium barbarum seed oil may reduce oxidative damage in testes of subacute senescent rats by activating the Nrf2/ARE signaling pathway.
